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Finally, soluble nuclear lysate was diluted with two volumes of the dilution buffer (60 mM HEPES-KOH (pH = 7.9), 1 mM DTT, 30 % glycerol, 0.4 % NP-40, 1 x phosphatase inhibitor cocktail (Sigma, P0044) and 1 x protease inhibitor cocktail (Sigma, P8340)) to lower KCL concentration to 150 mM

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Ongoing research is expected to expand the list of TCM-derived metabolites with evidence-based benefits, to optimize their formulations (for example, improving bioavailability of poorly absorbed molecules), and to clarify their roles in practice (whether as adjuncts to standard therapy to address residual risks like inflammation, or as alternatives in specific scenarios such as statin-intolerant patients)

Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging

Excessive ferroptosis is also closely related to autoimmune diseases, ovarian aging, polycystic ovary syndrome and other diseases (42, 83)