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Such difference may arise from the subtle changes in pocket residues at 3.37b (H268 for GLP-2R, Q234 for GLP-1R and Q232 for GCGR), 3.40b (V271 for GLP-2R, V237 for GLP-1R and I235 for GCGR) and ECL2 (N334 for GLP-2R, R299 for GLP-1R and N298 for GCGR)

Quality by Design (QbD) and Process Analytical Technology (PAT) ICH Q8(R2) defines Quality by Design as a systematic approach to development that begins with predefined objectives and emphasizes product and process understanding based on sound science and quality risk management

10.1016/s0166-2236(99)01401-0 Trends Neurosci