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Identification of TGR5 receptor-activated G proteins in STC-1 cells G proteins selectively activated by the TGR5 ligand were identified as described previously (Okamoto et al., 6 cells/ml) were homogenized in 20 mM HEPES medium (pH 7.4) containing 2 mM MgCl 2 , 1 mM EDTA, and 2 mM dithiothreitol

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Antagonist binds the receptor but does not activate it

While these results are suggestive of ferroptosis in this pathological scenario, further work using ferroptosis-specific inhibitors such as Fer-1 will be helpful to confirm this point