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In fact, the GSH/GSSG-Glo Assay can be performed using the same cell population in parallel wells during the 1.5-hour incubation period within the ApoTox-Glo Assay, an assay that assesses cell viability, cytotoxicity and apoptosis events in the same cell population by measuring a protease activity specific to live cells, a protease activity that is released from cells that have lost membrane integrity and caspase-3/7 activity

Testicular toxicity: evaluation during drug development

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It has to be mentioned that KO mice for IGF-1 gene are not viable and studies in such mice have to be done in the first days of life, and thus results are not very conclusive as metabolism is not properly established at this stage [129, 228]