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According to a prior research, increasing ROS by inhibiting GSH de novo synthesis but not recycling increased intracellular GSH production, which ultimately improved Treg cell differentiation and restricted Th17 cell differentiation (51)

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SH-SY5Y cells were seeded in 96-well plates at a density of ~ 5000/ per well, and they were exposed to various concentrations (10, 25, 50, 100, and 200 g/mL) of DNZ-NLPs for 24 h