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doi:10.1124/jpet.111.182220

NAION usually causes sudden, painless vision loss in one eye

Calcium assay in CHO-GLP-1R cells PathHunter GLP-1R cells were loaded with calcium dye (Calcium 6, Molecular Devices) in HBSS plus 20 mM HEPES and 2.5 mM probenecid 1 M agonist for 90 min at 37 C