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To rescue the Al 2 O 3 NPs induced mitochondria dysfunction, disruption of small molecular metabolites of HBE were profiled using metabolomics analysis, which facilitates identification of potential antagonizer or supplement against nanoparticle-involved damages

Proteins were denatured with 10 mM DTT and 8 M urea for 60 min, and then alkylated with 50 mM Iodoacetamide (IAA) for 30 min in the dark