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Carruthers, 2017) show no cumulative toxicity over a decade of treatment, no measurable atrophy of treated muscle beyond what reverses on discontinuation, and stable result reproducibility across product brands

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In deeply anaesthetised rats (placed in ventral position) the tail was transected with a surgical scalpel 3 cm from the tip (and submerged into a tube with 15 ml of saline at room temperature in vertical position) and the duration and amount of bleeding were measured to evaluate the hemostatic effect of the agents or saline administration