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After incubation, the beads were washed with 1 ml of ice-cold phosphate-buffered saline (PBS), pH 7.4, three times, and the beads were eluted in sample loading buffer by boiling for 10 min at 100 C

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The observed inhibitory effects of CitAgNPs are attributed to their ability interfere with certain gene regulation and inhibiting the translation process of amino acids, disrupting the production of exopolysaccharide layers essential for biofilm formation

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Tirzepatide: Well-characterized GI profile (nausea 12-33%, diarrhea 12-21%)