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Each assay was done in triplicate and each experiment was done three times

EPR spectroscopy Continuous wave EPR spectroscopy was performed at 20 C with a X-band (9.6346 GHz) spectrometer (EMX-Nano, Bruker Biospin, with a cylindric cavity mode TM1110)

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The increasing number of studies that are focused on TXN, TXNRD1, and TXNIP for the purpose of cancer therapy has further demonstrated the importance of the Trx system in the development of cancer Several studies have shown that the disruption of the Trx system can lead to increased oxidative stress, inflammation, and apoptosis, which are all associated with cancer progression

How often you should get glutathione IV therapy varies based on your individual goals