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3b and 4b) were fitted to a dose-dependent demetallation curve to determine the ligand concentration where the concentrations of copper ions bound to the protein was decreased by 50%

In a related area, studies are searching to find more effective methods of analyzing BBB permeability, which is often disrupted in many disease states

(b) the paracellular route used by ions, water and larger hydrophilic compounds from ~400600 Da up to 1020 kDa and that cannot cross transcellularly [regulated by the tight junction (TJ) proteins]

Cyanocobalamin (Vitamin B12) 4000mcg

Ferrioxamine and aluminoxamine are more stable complexes and are eliminated through the renal system (Kim et al ., 2019)