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10.2337/diabetes.53.3.654 [DOI] [PubMed] [Google Scholar] 16.Elliott RM, Morgan LM, Tredger JA, Deacon S, Wright J, Marks V

Alternatively, they may be tapered off over a period of time

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The distances migrated by individual bands of the standard proteins to the edge of the gel during electrophoresis are plotted against their respective pI values, thereby generating a standard curve

Until then, it's available only through clinical trial enrollment (free, pharmaceutical-grade, medically supervised), compounding pharmacies ($200$500/month with prescription), or research peptide vendors ($150$500/month, grey market)