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Western blotting Protein lysates were extracted from frozen RV tissue or cultured cardiomyocytes using RIPA buffer supplemented with protease and phosphatase inhibitors (Beyotime, China)

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Abstract Background Glutathione (GSH), a pivotal non-protein thiol, can be biosynthesized through three pathways in different organisms: (1) two consecutive enzymatic reactions catalyzed by -glutamylcysteine synthetase (Gsh1 or GshA) and glutathione synthetase (Gsh2 or GshB)

The conditioned medium from pancreatic cancer cells contained high IGFBP3 and caused significant myofiber wasting, which was prevented by IGFBP3 knockdown or neutralizing antibody [105]