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Cell viability and proliferation assays The transfected K-562 cells with or without cell sorting and the negative control cells transfected with empty plasmids pcDNA3.1-GST DAF or pcDNA3.1-GST CEAM7 were counted and seeded into 96-well plates in triplicates at a density of 1,000 per well

By reducing both the cleavage of caspase-3 itself and the cleavage of PARP, AHK-Cu appears to interfere with this terminal step in the apoptotic process

After further washing with TBST buffer, the membrane was photographed using the chemiluminescent imaging system