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If you see any, push the water back into the vial and draw again, or flick the syringe barrel gently to move bubbles to the top and push them out

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The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent