Then, three-dimensional fluorescence images of the cleared samples were obtained using a light sheet microscope (LiToneXL, Light Innovation Technology, China) equipped with a 4 objective lens (NA = 0.28), and thin light sheets were used to illuminate the four sides of the samples during imaging
All assays were run as per manufacturers recommendation, an overnight supernatant incubation protocol was used for all assays except Angiogenesis Panel 1 and Vascular Injury Panel 2, which were run according to the same day protocol
It has been shown that following a Mediterranean diet (the PREDIMED [Prevencin con DIeta MEDiterrnea] intervention) can lead to an increase in plasma total antioxidant capacity (or non-enzymatic antioxidant capacity, NEAC) (Zamora-Ros R et al., 2013)
Facial redness : Persistent redness or flushing on the cheeks, nose, forehead, and chin
Jan 2013;77(1):66-74