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Archived from the original on 12 August 2025

Olympus optical, Tokyo) for 30 min before recording

Following fixation in 4% paraformaldehyde (PFA), 5 dpf Brn3c:mGFP larvae, which express membrane-bound GFP in hair cells, were rinsed twice with phosphate-buffered saline (PBS) for 10 min each and then once with dH 2 o for 20 min

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It is the primary actin-sequestering protein in mammalian cells and has been studied for its role in cell migration, angiogenesis, and tissue repair