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Increased intima-media thickness and reduced nitrite levels in PCOS rats may contribute to hypertension (Jabbour et al

Cells were maintained at 37 C in 5% CO2/95% atmosphere and medium replaced every 3 days

Genes with fewer than 25 counts were excluded prior to differential expression analysis

The homogenizer was stopped and the solution was placed on a magnetic stirring plate, covered with parafilm and mixed with a magnetic stir bar until cooled to room temperature

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