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[ Acknowledgements We thank Sven Paul, Institute of Animal Breeding and Husbandry, University of Kiel, for revising the manuscript, Matthias Leippe, Department of Zoophysiology, University of Kiel, for helpful information and Virbac for providing the picture "Internal organs of the horse"

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DNA migration patterns were observed using a fluorescent microscope at 40 magnification and an excitation filter ranging from 420 to 490 nm

In this study, histopathological examination of both right sides in two groups was of great value not only in confirming the type of melasma but also adding another objective tool in evaluation of treatment response in two groups, it demonstrated a decrease in melanin in basal cell layer in epidermal types and in melanin and melanophages in dermis in mixed types in H and E and MF stained sections after treatment compared with baseline

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