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The average particle size of silver nanoparticles can be calculated using DebyeScherrer Equation (3): Where K is the Scherrer constant with value from 0.9 to 1 (shape factor), where is the X-ray wavelength (1.5418 ), 1/2 is the width of the XRD peak at half height and is the Bragg angle

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Then cells were diluted with 0.51 ml assay buffer, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)