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Cell culture and transient transfection The chicken Leghorn male hepatoma (LMH) cells were obtained from ATCC (Manassas, VA, USA)

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After the coverslips were floated off the sections, silver enhancement was performed using an HQ silver enhancement kit (Nanoprobes) for 3 min, and the organoids were prepared for electron microscopy as described previously 18,19

Kristina Young, M.D., Ph.D., was awarded a prestigious R01 grant from the National Institutes of Health (NIH), to pursue her project, Enhancing the efficacy of radiation by spatially restricting anti-TGF treatment to the tumor. Jianguo Huang, Ph.D., received a significant research grant from the Sarcoma Foundation of America for his study, Siglec15 Regulation of Immune Suppressive Microenvironment in Sarcoma. Providence Cancer Institute NCI Community Oncology Research Program (NCORP) earned the Gold Certificate of Excellence for our outstanding enrollment of patients in NCI trials