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Immunofluorescence Immunofluorescence staining was performed according to the standard protocol described previously ( Apoptosis Assay and TUNEL Assay Propidium iodide (PI) and Annexin V-FITC-flow cytometry assay (BD Pharmingen, CA) was used to detect the apoptosis in the cells

On one hand, the confidence intervals only allow for conclusions regarding non-inferiority, and on the other hand, the average duration of observation was only 1 year

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Waldstein said that because GLP-1 is a natural body hormone, making claims about side effect management is not ultra-high risk