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Patients are advised to follow sun protection and lifestyle recommendations to support treatment effectiveness

NCTC1469 cells, a normal mouse hepatocyte cell line, were obtained from the American Type Culture Collection and were cultured in DMEM containing 10% (v/v) fetal horse serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

Every patient is guided on: Daily broad-spectrum SPF 50+ Reapplication every 34 hours when outdoors Physical protection (caps, sunglasses, shade) Avoiding excessive heat exposure (steam, sauna, aggressive facials) during active treatment Consistent homecare to prevent rebound pigmentation International dermatology organisations such as the American Academy of Dermatology emphasise that strict sun protection is one of the most important factors in controlling pigmentation and melasma

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14 Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance