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The third site is composed from a free Cys34 residue and its surrounding and the fourth site with unknown location is called Site B

After being collected in 500 l of PBS the capillary fraction was divided into three aliquots, one for the maximum LDH release, one for the vehicle treatment induced LDH release (spontaneous LDH release) and one for the treatment induced LDH release

Further evidence for the hypothesis that glutathionylation is not supported by a suitable scaffold site in ROXY9 comes from the observation that Cys22 of the ROXY9-C 21 C 22 LS variant started to become glutathionylated at roughly the same redox potential as Cys21, albeit Cys22 is not oriented towards the GSH binding groove (Fig

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