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Gut hormonal analyses The Mesoscale Discovery (MSD, Rockville, MD, USA) U-PLEX Human Diabetes Combo 1 multiplex assay (K15274K) was used to perform analyses of C-peptide, GIP (total), glucagon-like peptide-1 (GLP-1,total), glucagon, insulin, leptin and PYY (total) in serum (fasting blood samples) and EDTA plasma (non-fasting blood samples) by electro-chemiluminescence detection

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For instance, coimmunoprecipitation cannot be used to examine interactions in living cells in real time

Vitamin B12 is crucial in energy production, nerve function, red blood cell formation, and metabolism