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The challenges of translating high-fat-fed rodents to human obesity and diabetes

doi: 10.1016/j.clnu.2014.03.003 134

[46] through a combination of coimmunoprecipitation, RNAi knockdown, immunoblotting, cAMP assays, and pharmacological assays demonstrated that -Arrestin-1 knockdown broadly attenuated GLP-1 signaling, causing decreased ERK and CREB activation and IRS-2 expression as well as reduced cAMP levels and impaired insulin secretion