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Blood 88(5):1747-54, 1996

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An assumption often made in aquatic chemosensory studies is that all animals randomly selected from a pool of individuals will respond to a stimulus (or make a choice upon exposure to multiple stimuli), underestimating the fact that very little information is available on the physiology, social status, and potential pre-exposure of the individuals tested

Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

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