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As COL IV genes are expressed by the animals body wall muscles in addition to their DTCs 44 , to avoid any confounding effects that could result from systemic manipulations of COL IV levels, we used a strain that allows us to specifically interfere with DTC COL IV production by RNAi

Get the math wrong and every subsequent step in the protocol carries an error either higher concentration than intended, lower, or an inconsistent dose across the study period

Paris J, McConahey WM, Tausie WN, Woolner LB, Bahn RC