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By optimizing skeletal muscle metabolism and counteracting metabolic decline, MOTS-c addresses the fundamental cellular machinery responsible for energy balance

TG, triglyceride

Interestingly, the overexpression of the individual protein isoforms in vitro results in distinct transcriptional regulatory profiles, with only about 10% overlap (28, 29)

In the brain, this process is predominantly mediated by microglia and astrocytes ( Paclitaxel-induced neuroinflammation is a key mechanism contributing to cognitive impairment in patients with cancer undergoing chemotherapy ( 4 Oxidative stress Oxidative stress occurs when the bodys production of ROS and free radicals exceeds its ability to neutralize them with antioxidants ( Increased ROS production is a common feature of chemotherapy-induced neurotoxicity ( 5 Impaired neurogenesis and synaptic plasticity The hippocampus is a critical brain region for learning and memory, and particularly vulnerable to paclitaxel-induced toxicity ( Few studies have investigated the effect of chemotherapy on synaptic plasticity

After 24 h incubation at 37 C, the cells were washed three times with PBS and fixed with 4% paraformaldehyde (Thermo Scientific, Waltham, Massachusetts, USA) for 10 minutes