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Prior to any procedures, animals were anesthetized using a combination of xylazine (10 mg/kg) and ketamine (90 mg/kg) for up to one hour via the intraperitoneal route

Despite their effectiveness, concerns remain regarding safety, tolerability and potential health risks, as well as the need for long-term administration and the subsequent cost

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Emission spectra were recorded every 5 min over a period of 100 min