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Another limitation is that using siblings as a comparison group, the age difference between the two groups can introduce some bias, and this must be taken into account in interpreting the results

Glu excitotoxicity results in excessive influx of extracellular Ca 2+ through the overstimulation of NMDAR, which may cause the release of Ca 2+ from intracellular stores, further increasing the concentration of cytosolic free Ca 2+ [46]

Cell Death Dis (2021) 12(7):696

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These experiments showed that blood plasma has NADH oxidizing activity, while cells have property to degrade added exogenous NADP + , NADPH and NAD +