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107 Although neutrophils and macrophages play an essential role in COPD development, an overactivated and dysregulated immune response can also cause inflammation and tissue damage

18 Sequence analysis Amino acid sequences were selected from NCBI and UniProtKB databases: Homo sapiens (code: P09211), Bos taurus (code: NP_803482.1), Capra hircus (code: Q9TTY8.2), Equus caballus (code: XP_001498156.1), Ovis aries (code: XP_004019771.1) and Sus scrofa (code: M3V836)

After washing twice with UA as above, 100 L of 50 mM AmBic (50 mM NH 4 HCO 3 in water) was added to the filter unit and the filter was centrifuged at 14,000 g for 10 min

The next day, the supernatant was decanted into a 15-mL sterile conical centrifuge tube and centrifuged at 400 g for 10 min at 4 C

[DOI] [PMC free article] [PubMed] [Google Scholar] 275.Yap S.P., Yuen K.H