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Reprogramming of glucose, fatty acid and amino acid metabolism for cancer progression

Traditional antidepressants usually take weeks to work

T.CelestinoI.CoppoL.HolmgrenA.et al

For binding assays, 100 g of each purified fusion protein was first immobilized on 50 l of packed glutathione-agarose beads at room temperature for 1 hour, the beads were washed once with cold homogenization buffer B, then 300 l of the diluted cytosol was added and the beads tumbled for 2 h at 4 C