To remove the ligands in the solution, the pellets were washed with PBS, centrifuged at 10,000 g for 30 min at 4 C, and re-suspended in the sonication buffer containing 50 mM HEPES (pH 7.4), 25 mM NaCl, 1 mM EDTA, 1 mM MgCl 2 , and 0.5% Triton X-100
There is no toxic dose established in humans
However, one day after exposure, the gap detection ratio of noise-exposed mice was significantly higher than that of the controls (Fig
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The following chapter examines how these physiological differences translate into psychological and behavioral processes during GLP-1 RA treatment