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(2019) suggested that the serum TMAO levels were positively correlated with the serum total bile acids concentrations, particularly the percentage of taurocholic acid (a farnesoid X receptor (FXR) antagonist bile acid), resulting in increased expression of bile acid synthesis enzyme CYP7A1 (cholesterol 7-hydroxylase) in NAFLD patients

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Organs were homogenized either by repetitively rolling a serological pipette over the samples in sample bags (liver) or by bead beating using a Precellys 24 Touch homogenizer (Bertin Technologies) at 5,000 r.p.m