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Subsequently, the proteins (20 ~ 80 g) were separated by 8%, 10%, or 12% sodium dodecyl sulfated-polyacrylamide gel electrophoresis using voltage mode ranging from 80 to 120 V, and then transferred to 0.22 m of polyvinylidene fluoride membranes (Millipore, Billerica, Mass., USA) using the Mini Trans-Blot electrophoretic transfer cell system (Bio-Rad Laboratories, Inc., Hercules, CA, USA)

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Therefore, the PI3K/AKT/mTOR pathway may exert organ-protective effects in S-ALI by regulating ferroptosis processes

(gynecologist), Mnica Aura Masip M.D., M.Sc

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