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Quantitative polymerase chain reaction was performed using pre-designed Taqman probes (Thermoscientific, USA) for rat GAPDH (Rn01775763), GLP-1R (Rn00562406_m1), CTR (Rn01526770_m1), RAMP1 (Rn01427056_m1), RAMP2 (Rn00824652_g1), RAMP3 (Rn00571815_m1), BDNF (Rn02531967_s1) and GDNF (Rn01402432_m1) transcripts

Examples may include the luteinizing hormone (LH), which is considered a crucial hormone in the regulation of reproductive hormones such as testosterone

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However, further research is necessary