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After centrifugation and thorough removal of the supernatant, the pellets were incubated for 1 h in 60 l staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 l anti-BrdUFITC monoclonal antibody (BioLegend, 364104, clone 3D4)
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In the brain, energy metabolism is tightly regulated as neurons are dependent on glucose as their primary energy source, but are unable to store sufficient quantities of glycogen to meet demand [118]
328 ADI-PEG-20, in combination with pemetrexed and cisplatin (ADIPEMCIS), was well tolerated in another phase I study of recurrent high-grade glioma (HGG) (NCT02029690)
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