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Abstract Background Non-communicable diseases (NCDs), including cardiovascular diseases, respiratory disorders, And age-related degenerative diseases, account for 74% of global mortality, imposing heavy burdens on healthcare systems and global sustainable development

Both modifications sit in the region that IGF binding proteins recognise, and the functional consequence is a large loss of IGFBP affinity while type 1 IGF receptor binding is largely preserved

Cells were washed and incubated with Cy-3 or Cy-5 conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA and Thermofisher)