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We deproteinized the heparinized plasma by mixing it with an equal volume of cold 5% aqueous 5-Sulfosalicylic Acid (SSA

We included only patients with at least 2 GLP-1 RA prescriptions given at least 6 months apart

Cleavage of the disulfide bonds Due to the high reactivity of -SH group, thiols present in plasma occur mainly in forms of symmetrical and mixed disulfides

Keeping detailed records of dosing, timing, and symptom changes helps optimize personal protocols over time

Body composition optimization Whether the goal is fat loss , muscle growth , or both simultaneously through recomposition protocols , pens simplify the daily execution