Hubbard WJ, Bland KI, Chaudry IH
After washes, bound antibodies were detected by chemiluminescence using SuperSignal substrate (Pierce) and a CLARIOstar reader (BMG LabTech)
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Several studies suggest that similar to lipids, reductions in food intake via intestinal protein in rodents are due to CCK release and subsequent activation of VANs, as (1) intestinal protein infusion increases CCK in rodents and humans 188,189
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