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Administration of MTX (5 mg/kg) to day 4 produced a significant decrease in hepatic glutathione (GSH) levels and a significant increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) liver enzymes, hepatic malondialdehyde (MDA), tumor suppressor protein (p53), Il-6 and IL-1 levels compared to the control group