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Immunofluorescence staining of oocytes After a brief rinse with PBS/0.05% polyvinylpyrrolidone (to prevent adhesion), the oocytes were permeabilized with 0.5% Triton X-100/PHEM for 5 min, fixed in 3.7% PFA/PHEM (18.14 g PIPES, 6.5 g HEPES, 3.8 g EGTA, 0.99 g MgSO4, pH 7.0 w/KOH) for 20 min, and washed three times (10 min each) with PBS/polyvinylpyrrolidone

On the other hand, UCs have received more ICI agents FDA approvals than many other cancers

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They combine broad-spectrum SPF with a hint of color to even out your skin tone and reduce the appearance of redness or blemishes, without feeling like makeup