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Fluorescence was assessed using a cytoFLEX cytometer (Beckman Coulter)

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The corpus luteum, that develops from an ovarian follicle during the luteal phase of the menstrual cycle, is initially responsible for the synthesis of these hormones but with the advancement of pregnancy their production is shifted from the corpus luteum to the placenta by the 7th9th week of gestation (Reference Tal and Taylor20)