semaglutide recombinant synthesis Total Synthesis of Semaglutide Based on a Soluble Hydrophobic-Support-Assisted Liquid-Phase Synthetic Method A Novel Method for Liraglutide

The present inventors after numerous trails and earnest efforts developed a process for the preparation of Semaglutide which involves less number of synthetic steps, simple operations and easy to carry out chemical conversions

Cooled the mixture to 25- 30C and stirred for 2 hr at same temperature

Wash buffer : 50mM Tris-HCl 20mM Immidazole, pH 8.0 0.1

The desired monocistronic (single copy) of His6-AV-Ubiquitin- semapeptide (nucleotide sequence)-encoding gene PCR product of ~320bp was obtained as evident by 1% (w/v) Agarose gel electrophoresis (Figure 1)

Cooled the reaction mixture to 25-30C, water (195 ml) was slowly added to it and stirred for 2 hr at the same temperature