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Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

By contrast, alcohol exposure tended to impose repressive marks on BDNF

Breeding of glutathione producing strain and study on shaking flask fermentation: M.C.E

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