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It's a complex picture, and more data is needed to fully understand these potential risks

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Methods Antibodies Anti-MBP tag mouse monoclonal antibody and anti c-Jun rabbit monoclonal antibody was used at 1:1000 dilution (Beyotime, AF2912, AF1612), anti-ubiquitin was used at 1:20000 dilution (Abcam, ab134953), anti-GST tag mouse monoclonal antibody was used at 1:5000 dilution (Yeason, 30901ES50), HRP-conjuagated FLAG-tag mouse monoclonal antibody was used at 1:1000 dilution (Yeason, 30502ES60)

You need to pre-load every syringe on day one

These data suggest that in the absence of GSH synthesis, the liver accumulates GSH precursors and can re-route them into alternative metabolic pathways