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The following reaction system was used: 1 ng pGEX4T-2 plasmid, 50 mM Tris-HCl (pH 7.8), 5 mM MgCl 2 , 10 mM 2-mercaptoethanol, 10 g/ml BSA, 1 ng forward primer FP1 and 1 ng reverse primer RP1, 20 M dNTP, 1 M dideoxynucleotides (ddNTP), 2 units DNA Polymerase I (Invitrogen), and ddH 2 O to the reaction volume of 100 l

doi:10.1186/s12872-025-04519-9 Kuebler U, Linnebank M, Semmler A, et al

However, as clinicians celebrate these advances, a Read More Beyond the FIB-4: Why Diabetes Care Is Becoming a Hepatology-Endocrinology Partnership May 20, 2026 Articles, Diabetes, Gastroenterology, Obesity, Obesity, Type 2 Diabetes, Type 2 Diabetes 1,761 views Screening for fatty liver disease in patients with diabetes is no longer limited to identifying elevated liver enzymes or calculating a fibrosis score during an annual visit