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Malmezat T, Breuille D, Capitan P, Mirand PP, Obled C

Chronic administration study Finally, to determine whether exendin-phe1 beta cell effects persist with chronic administration, we administered agonists continuously to HFHS-fed mice for 2 weeks via subcutaneous minipumps, using a relatively low dose of agonist (0.24 nmol kg 1 day 1 ) to reduce the weight-dependent effects

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Cells were next lysed with 3 mL of lysis buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 10% glycerol) for 1 h at 4 o C