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In addition, the high incidence of postoperative issues was likely due to the inclusion of obsolete bariatric surgery procedures, as well as some patients with PWS-specific conditions (i.e., abnormal pain threshold, inability to vomit, predisposition to acute gastric dilatation, or more severe preoperative clinical conditions)

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Reverse transcription was performed at 50C for 30 min, followed by an initial PCR activation step of 95C for 15 min

Glutathione IV therapy is generally very safe when carried out by qualified practitioners in a CQC-registered clinic

The ice packs were still cool on arrival, and the medication was at a safe temperature